CELL CULTURE SERVICES

Cell culture is a set of biology techniques used to grow cells outside their organism (ex-vivo) or their original environment, for the purpose of scientific experimentation.

The ICV-3C core facility of the Paris Brain Institute has all the equipment and techniques required for the culture of primary cells taken freshly from an organism, the culture of cell lines with a as well as organ slices.

Cortical, striatal, and hippocampal neurons culture

From murine embryonic neurons
Macroscopic dissection, plating, image analysis

Zebrafish cell culture

Dechorionation
Bilayer culture
Culture maintenance

Cell bank

4 cell types available:

NIH3T3
Hela
Cos7
HEK

Transfection / Lentiviral
infection and analysis

Transfection percentage
Viability

Organoid maintenance

Medium change
Cell count
Defusion

Immunofluorescence
on cells

Antibodies of interest
Intercalant

Mycoplasma tests

Contamination tests on culture supernatant based on mycoplasma activity

Training

User training on equipment for stand-alone use and scientific/technical assistance on demand
Training of students : cell culture protocol and equipment training

Workstation rental

Fully equipped culture boxes dedicated in L2 confinement:

Biological safety station (Hood Type II)
CO2 incubator
Cell culture small equipment (pipettes, pump, racks….)
Dedicated temperature-controlled space (+4,-20,-80°C)
Bench and space to stock
Access to a large stock of plastic and biological consumable
Access to common equipment and room (dark rooms for imaging operations, shared spaces for small equipment (centrifuge, water bath, scale) as well as large dissection rooms).