with 10 state-of-the-art technological platforms
The staff of the ICV-3C facility has the scientific and technical skills for your project implementation from sample preparation to data analysis and provides the necessary services and training.
It is possible to do theorical and practical training in the use of the MQ10 analyzer cytometer and the S3e cell sorter. The objective of this training is that all users quickly become autonomous and independent in the use of cytometers and have the necessary and sufficient theoretical bases to plan their cytometry project as well as possible.
Project development
- Welcoming and monitoring users
- Panel design
- Theoretical and experimental advice
Training
- Theoretical
- Using the:
- S3e Sorter
- MQ10 analyser
- Luminex MAGPIX
- Analysis (FlowJo, Kaluza & Flowlogic key available)
Experimental
- Types of studies:
- Cell viability
- Extra-, Intramembrane & Intranuclear, etc.
- Cytokine production
- Intracellular ion flow
- Proliferation / Cell cycle
- Apoptosis
- Labelling (limitation of aspecific labelling, titration, isotypes, etc.)
- Passage & sorting of samples (nuclei, cells, etc.)
- Making plates for the Luminex MAGPIX
- Plates run on the Luminex MAGPIX
Analysis
- Data formatting
- Support