with 10 state-of-the-art technological platforms
Cell culture is a set of biological techniques used to grow cells outside their organism (ex-vivo) or their original environment, for the purpose of scientific experimentation.
The ICV-3C core facility of the Paris Brain Institute has all the equipment and techniques required for the culture of primary cells taken freshly from an organism, the culture of cell lines with a as well as organ slices.
Cortical, striatal, and hippocampal neurons culture
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Zebrafish cell culture
- Dechorionation
- Bilayer culture
- Culture maintenance
Cell bank
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4 cell types available:
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Transfection / Lentiviral
infection and analysis
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Organoid maintenance
- Change in culture environment
- Cell count
- Defusion
Immunofluorescence
on cells
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Mycoplasma tests
- Contamination tests on culture supernatant based on mycoplasma activity
Training
- User training on equipment for stand-alone use and scientific/technical assistance on demand
- Training of students : cell culture protocol and equipment training
Workstation rental
Fully equipped culture boxes dedicated in L2 confinement:
Biological safety station (Hood Type II)- CO2 incubator
- Cell culture small equipments (pipettes, pump, racks….)
- Dedicated temperature-controlled space (+4,-20,-80°C)
- Bench and space to stock
- Access to a large stock of plastic and biological consumable
- Access to common equipments and room (dark rooms for imaging operations, shared spaces for small equipments (centrifuge, water bath, scale) as well as large dissection rooms).
It’s possible to rent just one work station or or more and therefore to have a complete dedicated box.
